≡

ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)

CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta

Specificity

This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1).
No significant cross-reactivity or interference between Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-91 81
EDTA plasma(n=5) 85-94 89
heparin plasma(n=5) 93-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-102% 78-101% 98-105% 95-105%
EDTA plasma(n=5) 92-99% 86-101% 85-103% 89-98%
heparin plasma(n=5) 85-94% 83-93% 95-102% 87-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
ActaMedicaMediterranea CIRCULATING MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (MIP1-Α) AS A POTENTIAL BIOMARKER FOR THE DIAGNOSIS OF PATIENTS WITH … 57e97ac908aeb34bc08fd41a.pdf
PLoS One Quantitative proteomics in A30P*A53T α-synuclein transgenic mice reveals upregulation of Sel1l. pubmed:28771510
Inflammation Research Leptin stimulates tissue rat mast cell pro-inflammatory activity and migratory response Pubmed:30019195
Pharmacological Reports Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without … Pubmed:29339257
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA026Hu01 Active Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Cell culture; Activity Assays.
RPA026Hu01 Recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA026Hu01 Polyclonal Antibody to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) WB; IHC; ICC; IP.
MAA026Hu22 Monoclonal Antibody to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) WB; IHC; ICC; IP.
SEA026Hu ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA026Hu CLIA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Chemiluminescent immunoassay for Antigen Detection.
LMA026Hu Multiplex Assay Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA026Hu01 ELISA Kit DIY Materials for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) Main materials for "Do It (ELISA Kit) Yourself".