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ELISA Kit for Hexosaminidase A Alpha (HEXa)

TSD; Hex-A; Tay Sachs Disease; GM2 Gangliosidosis; Beta-Hexosaminidase Subunit Alpha; Beta-N-Acetylhexosaminidase Subunit Alpha; N-Acetyl-Beta-Glucosaminidase Alpha

Specificity

This assay has high sensitivity and excellent specificity for detection of Hexosaminidase A Alpha (HEXa).
No significant cross-reactivity or interference between Hexosaminidase A Alpha (HEXa) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Hexosaminidase A Alpha (HEXa) and the recovery rates were calculated by comparing the measured value to the expected amount of Hexosaminidase A Alpha (HEXa) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-103 86
EDTA plasma(n=5) 96-104 101
heparin plasma(n=5) 96-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexosaminidase A Alpha (HEXa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexosaminidase A Alpha (HEXa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hexosaminidase A Alpha (HEXa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 89-105% 82-102% 79-101%
EDTA plasma(n=5) 92-101% 83-98% 95-103% 79-103%
heparin plasma(n=5) 92-105% 79-98% 87-105% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Neural Regen Res Functionality of a bicistronic construction containing HEXA and HEXB genes encoding ¦Â-hexosaminidase A for cell-mediated therapy of GM2 gangliosidoses 34100447
life Serum Cytokine Profile, Beta-Hexosaminidase A Enzymatic Activity and GM2 Ganglioside Levels in the Plasma of a Tay-Sachs Disease Patient after Cord Blood Cell … 34685379
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA195Mu01 Active Hexosaminidase A Alpha (HEXa) Cell culture; Activity Assays.
RPA195Mu01 Recombinant Hexosaminidase A Alpha (HEXa) Positive Control; Immunogen; SDS-PAGE; WB.
RPA195Mu02 Recombinant Hexosaminidase A Alpha (HEXa) Positive Control; Immunogen; SDS-PAGE; WB.
PAA195Mu01 Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) WB; IHC; ICC; IP.
PAA195Mu02 Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) WB; IHC; ICC; IP.
SEA195Mu ELISA Kit for Hexosaminidase A Alpha (HEXa) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA195Mu Multiplex Assay Kit for Hexosaminidase A Alpha (HEXa) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.