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ELISA Kit for Cathepsin K (CTSK)
CTS-K; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD; Pycnodysostosis; Cathepsin O; Cathepsin X
- Product No.SEA267Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
For more details, please contact local distributors! US$ 630 US$ 2835 US$ 5355 US$ 44100
Specificity
This assay has high sensitivity and excellent specificity for detection of Cathepsin K (CTSK).
No significant cross-reactivity or interference between Cathepsin K (CTSK) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cathepsin K (CTSK) and the recovery rates were calculated by comparing the measured value to the expected amount of Cathepsin K (CTSK) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-102 | 87 |
EDTA plasma(n=5) | 89-103 | 94 |
heparin plasma(n=5) | 78-94 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin K (CTSK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin K (CTSK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cathepsin K (CTSK) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-90% | 79-96% | 99-105% | 88-102% |
EDTA plasma(n=5) | 95-102% | 98-105% | 93-101% | 98-105% |
heparin plasma(n=5) | 80-101% | 91-104% | 91-103% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Acta Histochemica | MRMT-1 rat breast carcinoma cells and models of bone metastases: improvement of an in vitro system to mimic the in vivo condition. PubMed: S0065128112000773 |
Atherosclerosis.? | Biochemical and clinical correlation of intraplaque neovascularization using contrast-enhanced ultrasound of the carotid artery Pubmed:24534452 |
Genet. Mol. Res | Drynaria total flavonoids decrease cathepsin K expression in ovariectomized rats Pubmed:25036175 |
Age (Dordr) | In vitro method for the screening and monitoring of estrogen-deficiency osteoporosis by targeting peripheral circulating monocytes PubMed: 26250906 |
Journal of Infectious Diseases | Cathepsin K Contributes to Cavitation and Collagen Turnover in Pulmonary Tuberculosis PubMed: 26416658 |
PLoS One. | Effect of Vitamin D on Peripheral Blood Mononuclear Cells from Patients with Psoriasis Vulgaris and Psoriatic Arthritis pmc:PMC4822855 |
Cell Death And Differentiation | TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 Pubmed:29358671 |
Indian Journal of Dental Research | Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis Pubmed: 30409937 |
International Journal of Biological Macromolecules | A bioactive exopolysaccharide from marine bacteria Alteromonas sp. PRIM-28 and its role in cell proliferation and wound healing in vitro Pubmed: 30851325 |
毕业论文 | Biomarkers of Compromised Implant Fixation |
neuroscience letters | Compound K induces neurogenesis of neural stem cells in thrombin induced nerve injury through LXRα signaling in mice Pubmed: 32371156 |
Информационный алгоритм неинвазивной оценки остеорезорбтивного побочного действия глюкокортикостероидов у больных язвенным колитом | |
Особенности остеоиммунологических аспектов остеорезорбции при периимплантите, хроническом пародонтите и раке альвеолярного отростка и … |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
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