≡

ELISA Kit for Transforming Growth Factor Beta Receptor I (TGFbR1)

ACVRLK4; AAT5; ALK5; SKR4; TGFR-1; Activin A Receptor Type II-Like Kinase 53kDa; Activin receptor-like kinase 5; Serine/threonine-protein kinase receptor R4

Specificity

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta Receptor I (TGFbR1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta Receptor I (TGFbR1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta Receptor I (TGFbR1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta Receptor I (TGFbR1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biochem Pharmacol. Gallium, a promising candidate to disrupt the vicious cycle driving osteolytic metastases. pubmed:27378505
Scientific Reports TGF-β/BAMBI pathway dysfunction contributes to peripheral Th17/Treg imbalance in chronic obstructive pulmonary disease. pubmed:27549738
The Journal of Clinical Endocrinology & Metabolism Multiple soluble TGF-β receptors in addition to soluble endoglin are elevated in preeclamptic serum and they synergistically inhibit TGF-β signalling 10.1210/jc.2017-01150
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA397Mu01 Recombinant Transforming Growth Factor Beta Receptor I (TGFbR1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA397Mu02 Recombinant Transforming Growth Factor Beta Receptor I (TGFbR1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA397Mu01 Polyclonal Antibody to Transforming Growth Factor Beta Receptor I (TGFbR1) WB; IHC; ICC; IP.
PAA397Mu02 Polyclonal Antibody to Transforming Growth Factor Beta Receptor I (TGFbR1) WB; IHC; ICC; IP.
SEA397Mu ELISA Kit for Transforming Growth Factor Beta Receptor I (TGFbR1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA397Mu Multiplex Assay Kit for Transforming Growth Factor Beta Receptor I (TGFbR1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.