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ELISA Kit for Vitamin D Receptor (VDR)
NR1I1; Nuclear Receptor Subfamily 1 Group I Member 1; Calcitriol Receptor; 1,25-dihydroxyvitamin D3 receptor
- Product No.SEA475Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.233ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
For more details, please contact local distributors! US$ 630 US$ 2835 US$ 5355 US$ 44100
Specificity
This assay has high sensitivity and excellent specificity for detection of Vitamin D Receptor (VDR).
No significant cross-reactivity or interference between Vitamin D Receptor (VDR) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
6 | The vitamin D receptor: A therapeutic target for the treatment of breast cancer? Cgi: Content |
endocrine-related cancer | Vitamin D receptor as a target for breast cancer therapy. pubmed:28213567 |
Journal of Advanced Research | Effect of exercise on serum vitamin D and tissue vitamin Dreceptors in experimentally induced type 2 Diabetes Mellitus. pubmed:27504197 |
European Journal of Applied Physiology | Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain Pubmed:29143122 |
BBA- Molecular Basis of Disease | Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic … Pubmed: 30553018 |
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus |
Journal of Steroid Biochemistry and Molecular Biology | Status of vitamin D and the associated host factors in pulmonary tuberculosis patients and their household contacts: a cross sectional study Pubmed: 31255688 |
Scientific Reports | Association of Fok1 VDR polymorphism with Vitamin D and its associated molecules in pulmonary tuberculosis patients and their household contacts Pubmed: 31649297 |
Research square | Vitamin D Status in Dupuytren's Disease: Association with Clinical Status and Vitamin D Receptor Expression |
Betok Fish (Anabas testudineus) Oil Decreases Inflammatory Cytokine through Increasing Vitamin D Level in Rats-induced Systemic Lupus Erythematosus | |
iium medical journal malaysia | Positive Correlation between Monocyte-to-Lymphocyte Ratio and C-Reactive Protein in Vitamin D Deficient Preterm Infants with Respiratory Distress Syndrome |
Research Square | Identification of SNP of VDR and VDBP gene and its Dysregulated pathway through VDR-VDBP interaction network analysis in Vitamin D deficient Infertile … |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
APA475Hu01 | Active Vitamin D Receptor (VDR) | Cell culture; Activity Assays. |
RPA475Hu01 | Recombinant Vitamin D Receptor (VDR) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA475Hu01 | Polyclonal Antibody to Vitamin D Receptor (VDR) | WB |
MAA475Hu22 | Monoclonal Antibody to Vitamin D Receptor (VDR) | WB; IHC; ICC; IP. |
SEA475Hu | ELISA Kit for Vitamin D Receptor (VDR) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA475Hu | Multiplex Assay Kit for Vitamin D Receptor (VDR) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |