≡

ELISA Kit for Coagulation Factor IX (F9)

HEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B

Specificity

This assay has high sensitivity and excellent specificity for detection of Coagulation Factor IX (F9).
No significant cross-reactivity or interference between Coagulation Factor IX (F9) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Coagulation Factor IX (F9) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor IX (F9) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 83-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor IX (F9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 87-97% 80-90% 78-103% 78-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cardiovascular Research Loss of accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity Pubmed:29444200
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA840Ra01 Recombinant Coagulation Factor IX (F9) Positive Control; Immunogen; SDS-PAGE; WB.
PAA840Ra01 Polyclonal Antibody to Coagulation Factor IX (F9) WB; IHC; ICC; IP.
MAA840Ra21 Monoclonal Antibody to Coagulation Factor IX (F9) WB; IHC; ICC; IP.
SEA840Ra ELISA Kit for Coagulation Factor IX (F9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA840Ra Multiplex Assay Kit for Coagulation Factor IX (F9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.