≡

ELISA Kit for Gastrokine 1 (GKN1)

CA11; AMP18; BRICD1; FOV; Foveolin; 18 kDa antrum mucosa protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Gastrokine 1 (GKN1).
No significant cross-reactivity or interference between Gastrokine 1 (GKN1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Gastrokine 1 (GKN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Gastrokine 1 (GKN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-103 91
EDTA plasma(n=5) 81-103 88
heparin plasma(n=5) 83-92 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrokine 1 (GKN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrokine 1 (GKN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gastrokine 1 (GKN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-99% 90-103% 95-102% 90-97%
EDTA plasma(n=5) 85-97% 94-101% 98-105% 98-105%
heparin plasma(n=5) 83-92% 78-99% 80-101% 88-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Jnournal of Cell Biochemistry Gastrokine 1 regulates NF‐κB signaling pathway and cytokine expression in gastric cancers PubMed: 23444260
fedOA Searching for informative biomarkers for Gastric Cancer id:eprint
Journal of Negative Results in BioMedicine Gastrokine 1 mRNA in human sera is not informative biomarker for gastric cancer. pubmed:27452910
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC500Ra01 Recombinant Gastrokine 1 (GKN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC500Ra01 Polyclonal Antibody to Gastrokine 1 (GKN1) WB; IHC; ICC; IP.
MAC500Ra21 Monoclonal Antibody to Gastrokine 1 (GKN1) WB; IHC; ICC; IP.
SEC500Ra ELISA Kit for Gastrokine 1 (GKN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC500Ra Multiplex Assay Kit for Gastrokine 1 (GKN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.