≡

ELISA Kit for Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2)

NFAT1; NFATP; NF-ATP; NFAT pre-existing subunit; T-cell transcription factor NFAT1

Specificity

This assay has high sensitivity and excellent specificity for detection of Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2).
No significant cross-reactivity or interference between Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Pediatr Rheumatol Online J The elevated serum levels of calcineurin and nuclear factor of activated T-cells 1 in children with Kawasaki disease Pubmed: 32183825
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPL942Hu01 Recombinant Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) Positive Control; Immunogen; SDS-PAGE; WB.
PAL942Hu01 Polyclonal Antibody to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) WB; IHC; ICC; IP.
MAL942Hu21 Monoclonal Antibody to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) WB; IHC; ICC; IP.
SEL942Hu ELISA Kit for Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) Enzyme-linked immunosorbent assay for Antigen Detection.
LML942Hu Multiplex Assay Kit for Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.