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Carbonic Anhydrase II (CA2)

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CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C

Carbonic Anhydrase II (CA2)
CCC A782
HGNC 1373
MGI 88269
PubMed 16825953
RGD 2240
UniProt P00918
Wiki CA2

Carbonic anhydrase II (gene name CA2), is one of fourteen forms of humanαcarbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.

Carbonic anhydrase II is expressed at high levels in osteoclasts during bone resorption. Laitala and Vaananen (1994) demonstrated in 2 in vitro culture systems that antisense RNA and DNA blocked expression of CA II and led to decreased bone resorption. In the choroid plexus, carbonic anhydrase II supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA782Hu01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
NPA782Hu01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu02 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Hu04 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
APA782Hu01 Active Carbonic Anhydrase II (CA2) Cell culture; Activity Assays.
Antibodies PAA782Hu01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
MAA782Hu22 Monoclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Hu04 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Hu03 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Hu02 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Hu71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
Assay Kits SEA782Hu ELISA Kit for Carbonic Anhydrase II (CA2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NPA782Mu01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Mu01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA782Mu01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Mu71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
Assay Kits SEA782Mu ELISA Kit for Carbonic Anhydrase II (CA2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NPA782Ra01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Ra01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
APA782Ra01 Active Carbonic Anhydrase II (CA2) Cell culture; Activity Assays.
APA782Ra02 Active Carbonic Anhydrase II (CA2) Cell culture; Activity Assays.
Antibodies PAA782Ra01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
PAA782Ra02 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Ra71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
Assay Kits SEA782Ra ELISA Kit for Carbonic Anhydrase II (CA2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NPA782Rb01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA782Rb51 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Rb71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
PAA782Rb01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Carbonic Anhydrase II (CA2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Carbonic Anhydrase II (CA2) ELISA Kit Customized Service Offer

Organism species: Rhesus monkey (Simian)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA782Si01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
NPA782Si01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA782Si01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
MAA782Si22 Monoclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Si91 HRP-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
LAA782Si71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
PAA782Si02 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
MAA782Si21 Monoclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
Assay Kits SEA782Si ELISA Kit for Carbonic Anhydrase II (CA2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NPA782Ca01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Ca01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA782Ca01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Ca71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Carbonic Anhydrase II (CA2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Carbonic Anhydrase II (CA2) ELISA Kit Customized Service Offer

Organism species: Ovis aries; Ovine (Sheep)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins NPA782Ov01 Native Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA782Ov01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA782Ov01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
LAA782Ov71 Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Carbonic Anhydrase II (CA2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Carbonic Anhydrase II (CA2) ELISA Kit Customized Service Offer

Organism species: Chicken (Gallus)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA782Ga01 Recombinant Carbonic Anhydrase II (CA2) Positive Control; Immunogen; SDS-PAGE; WB.
APA782Ga01 Active Carbonic Anhydrase II (CA2) Cell culture; Activity Assays.
Antibodies PAA782Ga01 Polyclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
MAA782Ga21 Monoclonal Antibody to Carbonic Anhydrase II (CA2) WB; IHC; ICC; IP.
Assay Kits SEA782Ga ELISA Kit for Carbonic Anhydrase II (CA2) Enzyme-linked immunosorbent assay for Antigen Detection.
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  46. "Histidine --> carboxamide ligand substitutions in the zinc binding site of carbonic anhydrase II alter metal coordination geometry but retain catalytic activity."Biochemistry 36:15780-15791(1997) [PubMed] [Europe PMC] [Abstract]
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  50. "Mechanism of cyanamide hydration catalyzed by carbonic anhydrase II suggested by cryogenic X-ray diffraction."Biochemistry 39:12391-12397(2000) [PubMed] [Europe PMC] [Abstract]
  51. "Structural influence of hydrophobic core residues on metal binding and specificity in carbonic anhydrase II."Biochemistry 39:13687-13694(2000) [PubMed] [Europe PMC] [Abstract]
  52. "Structural and kinetic analysis of the chemical rescue of the proton transfer function of carbonic anhydrase II."Biochemistry 40:1741-1748(2001) [PubMed] [Europe PMC] [Abstract]
  53. "Fluoroaromatic-fluoroaromatic interactions between inhibitors bound in the crystal lattice of human carbonic anhydrase II."J. Am. Chem. Soc. 123:9620-9627(2001) [PubMed] [Europe PMC] [Abstract]
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  61. "Unexpected nanomolar inhibition of carbonic anhydrase by COX-2-selective celecoxib: new pharmacological opportunities due to related binding site recognition."J. Med. Chem. 47:550-557(2004) [PubMed] [Europe PMC] [Abstract]
  62. "Crystal structure of human carbonic anhydrase II at 1.95 A resolution in complex with 667-coumate, a novel anti-cancer agent."Biochem. J. 385:715-720(2005) [PubMed] [Europe PMC] [Abstract]
  63. "Structural and kinetic characterization of active-site histidine as a proton shuttle in catalysis by human carbonic anhydrase II."Biochemistry 44:1097-1105(2005) [PubMed] [Europe PMC] [Abstract]
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  75. "Carbonic anhydrase inhibitors: X-ray and molecular modeling study for the interaction of a fluorescent antitumor sulfonamide with isozyme II and IX."J. Am. Chem. Soc. 128:8329-8335(2006) [PubMed] [Europe PMC] [Abstract]
  76. "Carbonic anhydrase activators. Activation of isoforms I, II, IV, VA, VII, and XIV with L- and D-phenylalanine and crystallographic analysis of their adducts with isozyme II: stereospecific recognition within the active site of an enzyme and its consequences for the drug design."J. Med. Chem. 49:3019-3027(2006) [PubMed] [Europe PMC] [Abstract]
  77. "Carbonic anhydrase inhibitors: Hypoxia-activatable sulfonamides incorporating disulfide bonds that target the tumor-associated isoform IX."J. Med. Chem. 49:5544-5551(2006) [PubMed] [Europe PMC] [Abstract]
  78. "Carbonic anhydrase inhibitors: clash with Ala65 as a means for designing inhibitors with low affinity for the ubiquitous isozyme II, exemplified by the crystal structure of the topiramate sulfamide analogue."J. Med. Chem. 49:7024-7031(2006) [PubMed] [Europe PMC] [Abstract]
  79. "2-substituted estradiol bis-sulfamates, multitargeted antitumor agents: synthesis, in vitro SAR, protein crystallography, and in vivo activity."J. Med. Chem. 49:7683-7696(2006) [PubMed] [Europe PMC] [Abstract]
  80. "Saccharin inhibits carbonic anhydrases: possible explanation for its unpleasant metallic aftertaste."Angew. Chem. Int. Ed. Engl. 46:7697-7699(2007) [PubMed] [Europe PMC] [Abstract]
  81. "Atomic crystal and molecular dynamics simulation structures of human carbonic anhydrase II: insights into the proton transfer mechanism."Biochemistry 46:2930-2937(2007) [PubMed] [Europe PMC] [Abstract]
  82. "Speeding up proton transfer in a fast enzyme: kinetic and crystallographic studies on the effect of hydrophobic amino acid substitutions in the active site of human carbonic anhydrase II."Biochemistry 46:3803-3813(2007) [PubMed] [Europe PMC] [Abstract]
  83. "Carbonic anhydrase activators: L-Adrenaline plugs the active site entrance of isozyme II, activating better isoforms I, IV, VA, VII, and XIV."Bioorg. Med. Chem. Lett. 17:628-635(2007) [PubMed] [Europe PMC] [Abstract]
  84. "Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties."Bioorg. Med. Chem. Lett. 17:1726-1731(2007) [PubMed] [Europe PMC] [Abstract]
  85. "Carbonic anhydrase inhibitors: the X-ray crystal structure of the adduct of N-hydroxysulfamide with isozyme II explains why this new zinc binding function is effective in the design of potent inhibitors."Bioorg. Med. Chem. Lett. 17:2795-2801(2007) [PubMed] [Europe PMC] [Abstract]
  86. "Carbonic anhydrase inhibitors: inhibition of human, bacterial, and archaeal isozymes with benzene-1,3-disulfonamides -- solution and crystallographic studies."Bioorg. Med. Chem. Lett. 17:4201-4207(2007) [PubMed] [Europe PMC] [Abstract]
  87. "Carbonic anhydrase inhibitors. Interaction of the antiepileptic drug sulthiame with twelve mammalian isoforms: kinetic and X-ray crystallographic studies."Bioorg. Med. Chem. Lett. 17:4866-4872(2007) [PubMed] [Europe PMC] [Abstract]
  88. "Location of binding sites in small molecule rescue of human carbonic anhydrase II."Biophys. J. 92:562-570(2007) [PubMed] [Europe PMC] [Abstract]
  89. "Structural analysis of charge discrimination in the binding of inhibitors to human carbonic anhydrases I and II."J. Am. Chem. Soc. 129:5528-5537(2007) [PubMed] [Europe PMC] [Abstract]
  90. "Inhibition of carbonic anhydrase II by thioxolone: a mechanistic and structural study."Biochemistry 47:3174-3184(2008) [PubMed] [Europe PMC] [Abstract]
  91. "Role of hydrophilic residues in proton transfer during catalysis by human carbonic anhydrase II."Biochemistry 47:12028-12036(2008) [PubMed] [Europe PMC] [Abstract]
  92. "Carbonic anhydrase inhibitors. Interaction of 2-(hydrazinocarbonyl)-3-phenyl-1H-indole-5-sulfonamide with 12 mammalian isoforms: kinetic and X-ray crystallographic studies."Bioorg. Med. Chem. Lett. 18:152-158(2008) [PubMed] [Europe PMC] [Abstract]
  93. "Carbonic anhydrase inhibitors. Interaction of 2-N,N-dimethylamino-1,3,4-thiadiazole-5-methanesulfonamide with 12 mammalian isoforms: kinetic and X-ray crystallographic studies."Bioorg. Med. Chem. Lett. 18:999-1005(2008) [PubMed] [Europe PMC] [Abstract]
  94. "Carbonic anhydrase inhibitors. Interaction of indapamide and related diuretics with 12 mammalian isozymes and X-ray crystallographic studies for the indapamide-isozyme II adduct."Bioorg. Med. Chem. Lett. 18:2567-2573(2008) [PubMed] [Europe PMC] [Abstract]
  95. "Carbonic anhydrase inhibitors: the X-ray crystal structure of ethoxzolamide complexed to human isoform II reveals the importance of thr200 and gln92 for obtaining tight-binding inhibitors."Bioorg. Med. Chem. Lett. 18:2669-2674(2008) [PubMed] [Europe PMC] [Abstract]
  96. "Carbonic anhydrase inhibitors. Interaction of the antitumor sulfamate EMD 486019 with twelve mammalian carbonic anhydrase isoforms: Kinetic and X-ray crystallographic studies."Bioorg. Med. Chem. Lett. 18:4282-4286(2008) [PubMed] [Europe PMC] [Abstract]
  97. "Carbonic anhydrase inhibitors: binding of indanesulfonamides to the human isoform II."ChemMedChem 3:473-477(2008) [PubMed] [Europe PMC] [Abstract]
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