Packages (Simulation)

Polyclonal Antibody to Carbonic Anhydrase II (CA2)

Image (I)

DAB staining on IHC-P; Samples: Rabbit Colon Tissue; Primary Ab: 20µg/ml Cavia Anti-Rabbit CA2 Antibody Second Ab: 2µg/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody (Catalog: SAA544Gu09)
DAB staining on IHC-P;
Sample: Rabbit Colon Tissue
Primary Ab: 20µg/ml Cavia Anti-Rabbit CA2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody
(Catalog: SAA544Gu09)

Image (II)

Western Blot; Sample: Human 293T cell lysate; Primary Ab: 1µg/ml Cavia Anti-Rabbit CA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody (Catalog: SAA544Gu09)
Western Blot; Sample: Rabbit Liver lysate;
Primary Ab: 1µg/ml Cavia Anti-Rabbit CA2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody
(Catalog: SAA544Gu09)
Western Blot; Sample: Rabbit Spleen lysate;
Primary Ab: 1µg/ml Cavia Anti-Rabbit CA2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody
(Catalog: SAA544Gu09)
Western Blot; Sample: Rabbit Heart lysate;
Primary Ab: 1µg/ml Cavia Anti-Rabbit CA2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody
(Catalog: SAA544Gu09)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1µg/ml Cavia Anti-Rabbit CA2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody
(Catalog: SAA544Gu09)

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Instruction Manual

Polyclonal-Antibody-to-Carbonic-Anhydrase-II-(CA2)-PAA782Rb51.pdf

Certificate

Polyclonal Antibody to Carbonic Anhydrase II (CA2)

CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C

Product No.PAA782Rb51
Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostGuinea pig
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelAPC
Immunogen NPA782Rb01-Native Carbonic Anhydrase II (CA2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.46mg/ml
Organism Species MoreHomo sapiens (Human)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 103 239 US$ 239 US$ 342 US$ 855 US$ 3420
For more details, please contact local distributors!

SPECIFITY

The antibody is a cavia polyclonal antibody raised against CA2. It has been selected for its ability to recognize CA2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Proteomics Clin Appl.	Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. Pubmed:26970563
PROTEOMICS - Clinical Applications	Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509
Cell & Molecular Biology	Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … doi:10.1002

Catalog No.	Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species	Applications (RESEARCH USE ONLY!)
NPA782Rb01	Native Carbonic Anhydrase II (CA2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA782Rb01	Polyclonal Antibody to Carbonic Anhydrase II (CA2)	WB; IHC; ICC; IP.
PAA782Rb51	Polyclonal Antibody to Carbonic Anhydrase II (CA2)	WB; IHC; ICC; IP.
LAA782Rb71	Biotin-Linked Polyclonal Antibody to Carbonic Anhydrase II (CA2)	WB; IHC; ICC.