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Occludin (OCLN)

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Tight Junction Protein Occludin TM4 Minus

Occludin (OCLN)
CCC C228
HGNC 8104
MGI 106183
PubMed 8886979
RGD 620089
UniProt Q16625
Wiki Occludin

Occludin is a 65-kDa (504-amino acid polypeptide) integral plasma-membrane protein located specifically at tight junctions described for the first time in 1993 by S Tsukita.According to its overall hydrophilicity, occludin appears to span the plasma membrane four times, forming two extracellular loops and exposing its NH2 and COOH terminus to the cytosol.

Interaction of occludin with several cytoplasmic proteins of the junctional plaque has been found to occur via its COOH terminus, while the extracellular loops are thought to be involved in the regulation of paracellular permeability and cell adhesion. Phosphorylation/dephosphorylation plays a major role in regulation of occludin and tight junctions.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC228Hu01 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC228Hu01 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
MAC228Hu22 Monoclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
Assay Kits SEC228Hu ELISA Kit for Occludin (OCLN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC228Mu01 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu02 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu03 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu04 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu05 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC228Mu01 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
LAC228Mu71 Biotin-Linked Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC.
PAC228Mu02 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
Assay Kits SEC228Mu ELISA Kit for Occludin (OCLN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC228Ra01 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC228Ra01 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
MAC228Ra21 Monoclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
Assay Kits SEC228Ra ELISA Kit for Occludin (OCLN) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues."J. Cell Biol. 133:43-47(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Occludin confers adhesiveness when expressed in fibroblasts."J. Cell Sci. 110:1113-1121(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Splicing diversity of the human OCLN gene and its biological significance for hepatitis C virus entry."J. Virol. 84:6987-6994(2010) [PubMed] [Europe PMC] [Abstract]
  4. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Occludin TM4(-): an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain."J. Cell Sci. 115:3171-3180(2002) [PubMed] [Europe PMC] [Abstract]
  8. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  9. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions."J. Biol. Chem. 284:1559-1569(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Density-enhanced phosphatase 1 regulates phosphorylation of tight junction proteins and enhances barrier function of epithelial cells."J. Biol. Chem. 284:14997-15006(2009) [PubMed] [Europe PMC] [Abstract]
  12. "PKC eta regulates occludin phosphorylation and epithelial tight junction integrity."Proc. Natl. Acad. Sci. U.S.A. 106:61-66(2009) [PubMed] [Europe PMC] [Abstract]
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach."J. Proteome Res. 8:808-817(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria."Am. J. Hum. Genet. 87:354-364(2010) [PubMed] [Europe PMC] [Abstract]