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Recombinant Occludin (OCLN)

Tight Junction Protein Occludin TM4 Minus

  • Product No.RPC228Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass11.8kDa
  • Accurate Molecular Mass16kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe17~Ser107 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 168  US$ 420  US$ 840  US$ 2520  US$ 6300 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Brain Research HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9 ScienceDirect: S0006899311021408
Plos one Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation PubMed: PMC3688973
Journal of Neurotrauma A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adults PubMed: 25794137
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140
Sci Rep.  Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke articles:srep40331
Frontiers in Pharmacology Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … Pubmed:29375374
Thorax Fas activation alters tight junction proteins in acute lung injury Pubmed: 30385692
Antioxidants (Basel). The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets Pubmed: 31426309
DISEASE MARKERS Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumors Pubmed: 32884584
Burns Open Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA)
PLoS One A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … 34492054
CNS Neuroscience & Therapeutics Higher serum occludin after successful reperfusion Is associated with early neurological deterioration Pubmed:35338575
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC228Mu01 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu03 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu02 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu04 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC228Mu05 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC228Mu02 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
PAC228Mu01 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
LAC228Mu71 Biotin-Linked Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC.
SEC228Mu ELISA Kit for Occludin (OCLN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC228Mu Multiplex Assay Kit for Occludin (OCLN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.