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Monoclonal Antibody to Occludin (OCLN)

Tight Junction Protein Occludin TM4 Minus

  • Product No.MAC228Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 120  US$ 280  US$ 400  US$ 1000  US$ 4000 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against OCLN. It has been selected for its ability to recognize OCLN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Brain Research HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9 ScienceDirect: S0006899311021408
Plos one Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation PubMed: PMC3688973
Journal of Neurotrauma A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adults PubMed: 25794137
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140
Sci Rep.  Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke articles:srep40331
Frontiers in Pharmacology Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … Pubmed:29375374
Thorax Fas activation alters tight junction proteins in acute lung injury Pubmed: 30385692
Antioxidants (Basel). The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets Pubmed: 31426309
DISEASE MARKERS Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumors Pubmed: 32884584
Burns Open Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA)
PLoS One A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … 34492054
CNS Neuroscience & Therapeutics Higher serum occludin after successful reperfusion Is associated with early neurological deterioration Pubmed:35338575
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPC228Ra01 Recombinant Occludin (OCLN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC228Ra01 Polyclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
MAC228Ra21 Monoclonal Antibody to Occludin (OCLN) WB; IHC; ICC; IP.
SEC228Ra ELISA Kit for Occludin (OCLN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC228Ra Multiplex Assay Kit for Occludin (OCLN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.