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Active Urocortin (UCN)

UI; UROC; Urocortin 1; UCN1

ACTIVITY TEST

Urocortin (UCN) is a 40 amino acid peptide hormone that belongs to the corticotropin-releasing factor (CRF) family. It is expressed in specific regions of the brain but is also detectable in other organs notably the heart.UCN is involved in stress response and has diverse physiological functions including modulation of immune and inflammatory responses, as well as effects on the cardiovascular and central nervous systems.Besides,Urocortin 3 (UCN3) has been identified as an interactor of UCN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human UCN and recombinant mouse UCN3. Briefly, biotin-linked UCN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to UCN3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human UCN and recombinant mouse UCN3 was shown in Figure 1, the EC50 for this effect is 1.06ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Human Reproduction P-205 Basement membrane integrity is altered in the late secretary phase in women with endometriosis: implications for the pathogenesis of endometriosis OxfordJournals: source
PLOS ONE Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo Pubmed:25462164
Taiwan J Obstet Gynecol Novel markers in the diagnostics of endometriomas: Urocortin, ghrelin, and leptin or leukocytes, fibrinogen, and CA-125? PubMed: 25951715
Biomedical Journal of Scientific & Technical Research Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
Neurobiol Stress Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions 34527794
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA231Hu02 Recombinant Urocortin (UCN) Positive Control; Immunogen; SDS-PAGE; WB.
APA231Hu01 Active Urocortin (UCN) Cell culture; Activity Assays.
RPA231Hu01 Recombinant Urocortin (UCN) Positive Control; Immunogen; SDS-PAGE; WB.
CPA231Hu21 OVA Conjugated Urocortin (UCN) Immunogen; SDS-PAGE; WB.
PAA231Hu08 Polyclonal Antibody to Urocortin (UCN) WB; IHC; ICC; IP.
PAA231Hu01 Polyclonal Antibody to Urocortin (UCN) WB; IHC; ICC; IP.
LAA231Hu71 Biotin-Linked Polyclonal Antibody to Urocortin (UCN) WB; IHC; ICC.
MAA231Hu22 Monoclonal Antibody to Urocortin (UCN) WB; IHC; ICC; IP.
SEA231Hu ELISA Kit for Urocortin (UCN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA231Hu Multiplex Assay Kit for Urocortin (UCN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.