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Rat Model for Alzheimer Disease (AD)

Alzheimer's

  • Product No.DSI707Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceInduced by physical method and medicine
  • Model Animal StrainsSD Rats (SPF), healthy, male, body weight 250g~300g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
  • Modeling MethodSD rats were anesthetized with 1% pentobarbital sodium (40ug/g) intraperitoneal injection, then fixed on the stereotaxic apparatus. According to the rat stereotaxic apparatus map, bregma is origin and puncture is 3.5 mm behind the origin. Cut 2mm along the right side of the central line, open the harnpan with a dental drill, using a micro syringe insert 3mm from brain surface, (AP= -3.5 mm, ML=2.0 mm, DV=3.0 mm). Bilateral hippocampal CA1 region was injected with Aβ1-40 (10ug (1μL)) respectively, retain the needle 5min, then suture wound. 3 days after modeling, caudal vein injection of normal saline, drug given for 100μl/day last 21 days, behavior detection of Morris.
  • ApplicationsDiesase model
  • Downloadn/a
  • UOM Each case
  • FOB US$ 280 
    For more details, please contact local distributors!

Model Evaluation

Behavior detection:
21 days after drug administration, morris for all groups. The experiment is divided into place navigation test and spatial probe test.
1. Place navigation test: Rats were trained for 5 days, 4 times a day, 30min for each interval. Record the time it takes rats to find the platform from 4 entry points, i.e. incubation. The average score of the four incubation as the final result of the day entered the final statistics.
2. Spatial probe test: 6th days of the test, remove platform, from the furthest end of the platform into the water. Record the swimming track of rats within 30s, observe and analyse the residence time in the target quadrant and times to pass through the platform.
After behavior detection, remove brain in each group, peel the epencephal on ice, symmetrically cut the rest into halves. One half fixed in 4% paraformaldehyde for pathological examination and another takes the hippocampus for PCR and protein detection.

Pathological Results

1. Nissl's staining
Hippocampal fixed paraffin sections, Nissl staining, observation of the morphology and number of neurons in the hippocampal region. Under the light microscope, count and analyze the number of neurons at the same site of each group.
2. IF staining
IF staining, observation of Aβ1-40 staining in hippocampus. Under the fluorescence microscope, count and analyze the number of positive plaque at the same site of each group.
3. TUNEL staining
TUNEL staining, observation of neuronal apoptosis. Under the fluorescence microscope, count and analyze the number of positive staining at the same site of each group.

Cytokines Level

1. RT-qPCR
Collect hippocampal tissue, extract RNA, reverse transcribe cDNA, detect expression of caspase-3, Bcl-2 and Bax by PCR.
2. Western-blot
Collect hippocampal tissue, detect protein expression of active-caspase-3, Bcl-2 and Bax.

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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DSI707Ra01 Rat Model for Alzheimer Disease (AD) Diesase model