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Rat Model for Dystonia (DT)

  • Product No.DSI839Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceInduced by 3- nitropropionic acid (3-NP)
  • Model Animal StrainsSD rats(SPF class), healthy, 6~8W, body weight:180g-200g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high)
  • Modeling Period2~4w
  • Modeling Method1. Modeling and identification of dystonia in rats
    1.1 Modeling method
    The experiment was conducted after adaptive feeding of male SD rats for a week .After anesthesia, the rats in the model group were fixed on the stereotaxic instrument. The coordinates of the left caudate putamen were determined to be 1mm of bregma, 3mm on the left side of the sagittal suture, and ~7mm of dura mater. Routine disinfection was performed, the skin and subcutaneous tissues of the head were cut, the outer membrane of the skull was bluntly separated, the anterior fontanelle and the left skull were fully exposed, and the skull was drilled open (be careful not to damage the dura). 3-NP solution (1000nmol/ul) was injected into the target with a microsyringe, 4ul per injection. 4~7 points were injected subdural, and the needle was left for 5 minutes after the injection, and the needle was withdrawn slowly. The control group was injected with normal saline at the same site. The animals were routinely fed in the same environment after operation.
  • ApplicationsDisease Model
  • Downloadn/a
  • UOM Each case
  • FOB US$ 240 
    For more details, please contact local distributors!

Model Evaluation

1.Flat score:
Before modeling, all animals were trained on a flat: a narrow flat, 120cm long and 7cm wide, was placed between two platforms 1m high from the ground. All rats were trained 3 times a day for 7 days to ensure that each rat could successfully pass the flat plate without stopping in between. The time and number of steps taken by each rat through the tablet were recorded to calculate stride length.
Five days after the operation, animals in each group were subjected to the flat test, and the time and number of steps of each rat were recorded to calculate the stride length.

2.Motor coordination test:
The motor coordination ability of rats was quantified by rotarod test.The rats' stay time on the rotating rod was tested at 9r/min and 18r/min respectively. Each animal was measured three times at each rotating speed and the average value was taken.
3.Take sample and detection:
The rats were sacrificed 7 days after operation for brain tissue and serum samples.

Pathological Results

HE staining and Nissl staining:
Brain tissue was taken, embedded and sected, and HE staining and Nissl staining were performed to observe pathological changes, mainly observing the changes of neurons in striatum, midbrain and hippocampus.

Cytokines Level

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
DSI839Ra01 Rat Model for Dystonia (DT) Disease Model