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Rat Model for Stress Urinary Incontinence (SUI)

Pelvic Floor Dysfunction； Bladder incontinence stress；Urinary leakage

  • Product No.DSI843Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceMechanical dilation of vagina and ligation of bilateral vulva nerves
  • Model Animal StrainsSD Rats(SPF), pregnant , female, body weight 290g~350g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group (Tiapride Hydrochloride )and Test drug group (three doses)
  • Modeling Period8 weeks
  • Modeling MethodConstruction of stress urinary incontinence (a rat model with impaired pelvic floor function)

    Modeling: Mechanical dilation of the vagina and ligation of bilateral pudendal nerves in postpartum rats to create an animal model simulating human delivery.

    Modeling methods: 12-week-old female Wistar rats (290-300 g) and adult male rats (290-300 g) were adapted to feeding for 1W, respectively. Then caged together by 2:1.One day after the cage was closed, the vaginal plug was tested, and the rats were taken out and raised alone. About 2w later, the pregnant female rats were taken out, and 1 day after the first delivery, they were randomly divided into two groups: control group and model group.

    Group ⅰ was the control group.

    Group ⅱ was vaginal dilation group, simulated birth injury

    1.1 Vaginal dilation: Chloral hydrate intraperitoneal injections of anesthesia, 8 F Foley catheter (disposable with balloon catheter) in rat vaginal head end, take 5 mL saline with a syringe, part into the air, observation of rat pubic symphysis above have airbags, from the vagina and adjust the catheter insertion depth above the pubic symphysis, confirmed the conformity of pubic symphysis place, 5 mL saline was fully injected into the catheter, 150 mg weight was hung at the tail end, and the rats were placed on the horizontal table, so that the sympubis was tangent to the edge of the table (pay attention to avoid the catheter touching the table, and keep the pulley and vagina at the same height during the traction process, so that the traction force could be fully applied to the vaginal wall). After maintaining this state for 4 h, the water sac was slowly released, and a small amount of sterile normal saline was injected along the vaginal opening, and then the urinary canal was removed. This method was used to simulate the prolongation of the second labor course during delivery, and the fetus produced long-term compression on the pelvic floor.

    1.2 Bilateral pubic nerve dissection: After female rats were anesthetized and fixed, the dorsal midline skin incision and bilateral dorsal muscle incision were taken, identified at the sciatic rectum fossa under the microscope, and the pudendal nerve was separated. The two sides of the pudendal nerve were separated at the obturator muscle branch by 0.3-0.5cm with minimally invasive surgical forceps, and then the wounds were closed. The postoperative model of bilateral pudendal nerve dissociation was established by prophylactic antibiotics. The control group received the same sham operation without bilateral pudendal nerve dissociation.
  • ApplicationsDisease Model
  • Downloadn/a
  • UOM Each case
  • FOB US$ 300 
    For more details, please contact local distributors!

Model Evaluation

Sneeze experiment: the urethral orifice was disinfected with iodophor, and the epidural catheter coated with paraffin oil was inserted into the bladder through the urethra and placed about 2 ~ 3 cm. After the bladder was emptied, the syringe was connected with the epidural catheter, and the sterile normal saline stained with Meilan was slowly injected into the bladder through the syringe, and the maximum bladder capacity was recorded when blue fluid spilled from the urethral orifice. After the bladder was emptied again, sterile normal saline with methylene blue staining was injected into the maximum bladder capacity. A section of rat whiskers was cut and extended into the nostrils of the rats to induce sneezing reflex. After careful observation of the urethral orifice of rats, blue liquid flowing out of the urethral orifice was regarded as positive for sneezing test, while no blue liquid flowing out of the urethral orifice was regarded as negative. The number of rats with positive sneezing test was calculated.

Pathological Results

Detection: Rats were sacrificed by neck amputation method. After lengthening the midabdominal line incision, a transverse incision was made on both sides of the bottom respectively. After exposing subcutaneous tissue, fat and tissue were bluntly separated to reveal symphysis pubis. After cutting open the symphysis pubis with scissors, expose the bladder neck and the upper middle part of the urethra, and remove the urethra and the anterior wall of the vagina in one piece.

Pathological examination: HE staining, Masson staining and CD31 (immunohistochemistry).

Cytokines Level

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
DSI843Ra01 Rat Model for Stress Urinary Incontinence (SUI) Disease Model
TSI843Ra81 Rat Pelvic floor muscle Tissue of Stress Urinary Incontinence (SUI) Paraffin slides for pathologic research: IHC,IF and HE,Masson and other stainings