Eukaryotic Collagen Type I Alpha 1 (COL1a1)
COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
- Product No.EPA350Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceEukaryotic expression
- Host293F cell
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationExtracellular matrix
- Predicted Molecular Mass29.1kDa
- Accurate Molecular Mass35kDa(Analysis of differences refer to the manual)
- Residues & TagsAsp1219~Leu1464 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.0
-
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 234
For more details, please contact local distributors! US$ 585 US$ 1170 US$ 3510 US$ 8775
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- BCA Protein Quantification Kit
- Protein Labeling Customized Service
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology | Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro study ScienceDirect: S1079210410004919 |
Journal of Biomedical Materials | Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study Wiley: source |
European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847 |
J Voice. | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429 |
Arch Oral Biol. | Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Pubmed: 24370188 |
Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625 |
UNIVERSA MEDICINA | Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture Univmed:Source |
International Journal of Neuropsychopharmacology | The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. pubmed:27477355 |
National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396 |
Nephron. | Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor pubmed:28768285 |
Cell and Tissue Research | Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and … Pubmed:29637306 |
International Journal of Molecular Medicine | LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway Pubmed: 30320382 |
International Journal of Molecular Sciences | Association between Plasma HMGB-1 and Silicosis: A Case-Control Study Pubmed: 30558126 |
Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945 |
Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response |
Scientific reports | Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells 33911160 |
Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677 |