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Human Jurkat cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human HDAC1 Monoclonal Antibody (Catalog FAC206Hu08, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Anti-Histone Deacetylase 1 (HDAC1) Monoclonal Antibody
HDAC1; GON10; HD1; RPD3; RPD3L1
- Product No.FAC206Hu08
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)E5
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
US$ 132
US$ 220
US$ 352
US$ 440
US$ 770
For more details, please contact local distributors!
USAGE
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Integrative Medicine | Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase Pubmed:25609369 |
| Tzu Chi Medical Journal | Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cells Science: Article |
| Restor Neurol Neurosci | Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal rats PubMed: 26410212 |
| Pharmacol Biochem Behav | Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's disease PubMed: 25931268 |
| 网页打不开 | Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cells S1016319016300295 |