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High Sensitive ELISA Kit for Heat Shock Protein 60 (Hsp60)
HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein
- Product No.HEA822Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 32pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 485
US$ 693
US$ 3119
US$ 5891
US$ 48510
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of High Sensitive Heat Shock Protein 60 (Hsp60).
No significant cross-reactivity or interference between High Sensitive Heat Shock Protein 60 (Hsp60) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Heat Shock Protein 60 (Hsp60) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Heat Shock Protein 60 (Hsp60) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-89 | 86 |
| EDTA plasma(n=5) | 92-103 | 101 |
| heparin plasma(n=5) | 80-104 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Heat Shock Protein 60 (Hsp60) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Heat Shock Protein 60 (Hsp60) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Heat Shock Protein 60 (Hsp60) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-92% | 85-96% | 99-105% | 97-105% |
| EDTA plasma(n=5) | 84-99% | 81-103% | 90-99% | 87-104% |
| heparin plasma(n=5) | 79-103% | 97-105% | 84-104% | 84-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Sci Rep | Hsp60 response in experimental and human temporal lobe epilepsy PubMed: 25801186 |
| Cellular and Molecular Gastroenterology and Hepatology | Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro Pubmed: 30878663 |
| Breast Cancer Res Treat | Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970 |