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ELISA Kit DIY Materials for Mucin 2 (MUC2)
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal
- Product No.KSA705Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.78-50ng/mL
- Applicable Sensitivity0.30ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1166
US$ 1944
US$ 3499
US$ 6804
US$ 11664
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 2 (MUC2). No significant cross-reactivity or interference between Mucin 2 (MUC2) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734 |
| World J Gastroenterol. | Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. Pubmed:24627600 |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis PubMed: 26217323 |
| nutrition research and practice | Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway PubMed: 26244071 |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851 |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513 |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489 |
| Bio-protocol | Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA 10.21769:BioProtoc.2879 |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494 |
| Biomolecules | Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer Pubmed: 30682816 |
| Cell Stem Cell | PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism Pubmed: 31564549 |
| DIGESTIVE DISEASES AND SCIENCES | Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats Pubmed: 32144600 |
| Scientific Reports | Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli Pubmed: 32601325 |
| Biorxiv | Defective intestinal repair after short-term high fat diet due to loss of efferocytosis |
| J Cell Mol Med | Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis 34145945 |
| Cell Tissue Res | Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced … 34370080 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA705Mu02 | Recombinant Mucin 2 (MUC2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA705Mu03 | Recombinant Mucin 2 (MUC2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA705Mu01 | Recombinant Mucin 2 (MUC2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA705Mu02 | Polyclonal Antibody to Mucin 2 (MUC2) | WB; IHC; ICC; IP. |
| PAA705Mu03 | Polyclonal Antibody to Mucin 2 (MUC2) | WB; IHC; ICC; IP. |
| PAA705Mu01 | Polyclonal Antibody to Mucin 2 (MUC2) | WB; IHC |
| LAA705Mu81 | FITC-Linked Polyclonal Antibody to Mucin 2 (MUC2) | WB; IHC; ICC; IF. |
| LAA705Mu71 | Biotin-Linked Polyclonal Antibody to Mucin 2 (MUC2) | WB; IHC; ICC. |
| SEA705Mu | ELISA Kit for Mucin 2 (MUC2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA705Mu | Multiplex Assay Kit for Mucin 2 (MUC2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA705Mu01 | ELISA Kit DIY Materials for Mucin 2 (MUC2) | Main materials for "Do It (ELISA Kit) Yourself". |