ELISA Kit for Mucin 2 (MUC2)

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

Specificity

This assay has high sensitivity and excellent specificity for detection of Mucin 2 (MUC2).
No significant cross-reactivity or interference between Mucin 2 (MUC2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 2 (MUC2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 2 (MUC2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734
World J Gastroenterol. Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. Pubmed:24627600
Front Microbiol Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis PubMed: 26217323
nutrition research and practice Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway PubMed: 26244071
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
Bio-protocol Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA 10.21769:BioProtoc.2879
Journal of Investigative Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494
Biomolecules Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer Pubmed: 30682816
Cell Stem Cell PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism Pubmed: 31564549
DIGESTIVE DISEASES AND SCIENCES Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats Pubmed: 32144600
Scientific Reports Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli Pubmed: 32601325
Biorxiv Defective intestinal repair after short-term high fat diet due to loss of efferocytosis
J Cell Mol Med Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis 34145945
Cell Tissue Res Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced … 34370080
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