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ELISA Kit DIY Materials for Tyrosine Hydroxylase (TH)

TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14

  • Product No.KSB438Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.066ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH). No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Mol Neurobiol. 1, 25-dyhydroxyvitamin D3 Attenuates l-DOPA-Induced Neurotoxicity in Neural Stem Cells Pubmed:25102940
PLOS ONE Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing Pubmed:25181484
biomedicine & pharmacotherapy Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. pubmed:28199883
Therapeutics and Clinical Risk Management Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. pubmed:27540296
Sci Rep.  Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. pubmed:28230187
Journal of Cellular Biochemistry Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive rats pubmed:28681939
Environmental Science & Technology Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate Pubmed:29565584
Molecular Neurobiology Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model Pubmed:29429051
Chemosphere Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae Pubmed:29407805
Neurospine The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells Pubmed:29656620
Toxicology and Applied Pharmacology Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects Pubmed: 30222980
International Journal Of Cardiology Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus Pubmed: 30581101
Behavioural Brain Research Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition
Environment International Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruption Pubmed: 31733528
Environmental Pollution Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae Pubmed: 31935612
International Journal of Pharmacology Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease Pubmed: 32084576
comparative biochemistry and physiology c-toxicology & pharmacology Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvae Pubmed: 32344129
International Journal of Stem cell Research & Therapy Selection of Cells for Parkinson's Disease Cell-Therapy
Ecotoxicol Environ Saf Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae 33385677
Nutr Res Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and … Pubmed:35065349
Food Chem Toxicol Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation Pubmed:35439590
Food Chemistry: Molecular Sciences Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approaches Pubmed:35415676
Journal of Environmental Sciences Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB438Hu01 Recombinant Tyrosine Hydroxylase (TH) Positive Control; Immunogen; SDS-PAGE; WB.
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KSB438Hu01 ELISA Kit DIY Materials for Tyrosine Hydroxylase (TH) Main materials for "Do It (ELISA Kit) Yourself".