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ELISA Kit DIY Materials for Hedgehog Homolog, Indian (IHH)

BDA1; HHG2

  • Product No.KSD116Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.062ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Hedgehog Homolog, Indian (IHH). No significant cross-reactivity or interference between Hedgehog Homolog, Indian (IHH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
The Journal of Biological Chemistry Prostate cancer cells and bone stromal cells mutually interact with each other through bone morphogenetic protein-mediated signals PubMed: 22532569
Cancer Letters Defective CFTR promotes intestinal proliferation via inhibition of the hedgehog pathway during cystic fibrosis Pubmed: 30639531
Journal of Hepatology Eugenia Marbach-Breitrück, Madlen Matz-Soja, Ute Abraham, Wolfgang Schmidt-Heck, Susanne Sales, Christiane Rennert, Matthias Kern, Susanne Aleithe, Luise?… Pubmed: 30711403
Tick-Tock hedgehog–mutual crosstalk with liver circadian clock renders hepatic hedgehog signaling a risk factor of steatosis
Molecular Medicine Reports MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression Pubmed: 32705199
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD116Ra01 Recombinant Hedgehog Homolog, Indian (IHH) Positive Control; Immunogen; SDS-PAGE; WB.
PAD116Ra01 Polyclonal Antibody to Hedgehog Homolog, Indian (IHH) WB; IHC; ICC; IP.
MAD116Ra21 Monoclonal Antibody to Hedgehog Homolog, Indian (IHH) WB; IHC; ICC; IP.
SED116Ra ELISA Kit for Hedgehog Homolog, Indian (IHH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD116Ra Multiplex Assay Kit for Hedgehog Homolog, Indian (IHH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD116Ra01 ELISA Kit DIY Materials for Hedgehog Homolog, Indian (IHH) Main materials for "Do It (ELISA Kit) Yourself".