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Monoclonal Antibody to Hedgehog Homolog, Indian (IHH)

BDA1; HHG2

  • Product No.MAD116Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 120  US$ 280  US$ 400  US$ 1000  US$ 4000 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against IHH. It has been selected for its ability to recognize IHH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The Journal of Biological Chemistry Prostate cancer cells and bone stromal cells mutually interact with each other through bone morphogenetic protein-mediated signals PubMed: 22532569
Cancer Letters Defective CFTR promotes intestinal proliferation via inhibition of the hedgehog pathway during cystic fibrosis Pubmed: 30639531
Journal of Hepatology Eugenia Marbach-Breitrück, Madlen Matz-Soja, Ute Abraham, Wolfgang Schmidt-Heck, Susanne Sales, Christiane Rennert, Matthias Kern, Susanne Aleithe, Luise?… Pubmed: 30711403
Tick-Tock hedgehog–mutual crosstalk with liver circadian clock renders hepatic hedgehog signaling a risk factor of steatosis
Molecular Medicine Reports MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression Pubmed: 32705199
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD116Ra01 Recombinant Hedgehog Homolog, Indian (IHH) Positive Control; Immunogen; SDS-PAGE; WB.
PAD116Ra01 Polyclonal Antibody to Hedgehog Homolog, Indian (IHH) WB; IHC; ICC; IP.
MAD116Ra21 Monoclonal Antibody to Hedgehog Homolog, Indian (IHH) WB; IHC; ICC; IP.
SED116Ra ELISA Kit for Hedgehog Homolog, Indian (IHH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD116Ra Multiplex Assay Kit for Hedgehog Homolog, Indian (IHH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD116Ra01 ELISA Kit DIY Materials for Hedgehog Homolog, Indian (IHH) Main materials for "Do It (ELISA Kit) Yourself".