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Western Blot; Sample: Mouse Heart lysate
Primary Ab: 0.2µg/ml Biotin-Linked Polyclonal Antibody to Cardiac Troponin I (cTnI)
Second Ab: 0.2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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Biotin-Linked Polyclonal Antibody to Cardiac Troponin I (cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
- Product No.LAA478Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 92
US$ 216
US$ 308
US$ 770
US$ 3080
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against cTnI. It has been selected for its ability to recognize cTnI in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
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| canadian journal of physiology and pharmacology | Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? Pubmed: 30840489 |
| Journal of Cellular Physiology | Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes Pubmed: 30982981 |
| BIOSENS BIOELECTRON | Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA … Pubmed: 30954926 |
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| Pharmacol Res | Therapeutic potential of cPLA2 inhibitor to counteract dilated-cardiomyopathy in cholesterol-treated H9C2 cardiomyocyte and MUNO rat Pubmed: 32942017 |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial Infarction in a Preclinical Porcine Model of Ischemic Heart Disease Pubmed: 32605184 |
| Eur J Pharmacol | NLRX1 knockout aggravates lipopolysaccharide (LPS)-induced heart injury and attenuates the anti-LPS cardioprotective effect of CYP2J2/11, 12-EET by enhancing … Pubmed: 32574674 |
| American Journal of Translational Research | Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and … Pubmed: 32509190 |
| Arabian Journal for Science and Engineering | Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes | |
| Hum Exp Toxicol | Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats 33882726 |
| Curr Protoc | Isolation and Culture of Neonatal Murine Primary Cardiomyocytes 34289259 |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species 34536344 |
| Biomed Pharmacother | Pea (Pisum sativum) peel extract attenuates DOX-induced oxidative myocardial injury 34649330 |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications 34673993 |
| Biomed Res Int | Nicotinic Acid Riboside Regulates Nrf-2/P62-Related Oxidative Stress and Autophagy to Attenuate Doxorubicin-Induced Cardiomyocyte Injury Pubmed:35242876 |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein Pubmed:35714415 |
| Nutrients | Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 Pubmed:35215383 |