Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1)

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

Product No.LAB449Hu71
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceAntibody labeling
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelBiotin
Original Antibody n/a
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
Downloadn/a
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 110 256 US$ 256 US$ 366 US$ 915 US$ 3660
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Analytical Chemistry	Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor PubMed: 20557064
Translational Research	Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385
Journal of Proteomics	Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921
BioMed Research International	The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis Pubmed: 33062681
Cancer Cell International	Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
Cancer Cell Int	Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212
Clin Proteomics	Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma Pubmed:35610567

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB449Hu03	Recombinant Enolase 1 (ENO1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB449Hu02	Recombinant Enolase 1 (ENO1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB449Hu01	Recombinant Enolase 1 (ENO1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB449Hu02	Polyclonal Antibody to Enolase 1 (ENO1)	WB; IHC; ICC; IP.
PAB449Hu03	Polyclonal Antibody to Enolase 1 (ENO1)	WB; IHC; ICC; IP.
PAB449Hu01	Polyclonal Antibody to Enolase 1 (ENO1)	WB; IHC; ICC; IP.
LAB449Hu71	Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1)	WB; IHC; ICC.
MAB449Hu21	Monoclonal Antibody to Enolase 1 (ENO1)	WB; IHC; ICC; IP.
MAB449Hu22	Monoclonal Antibody to Enolase 1 (ENO1)	WB; IHC.
SEB449Hu	ELISA Kit for Enolase 1 (ENO1)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEB449Hu	ELISA Kit for Anti-Enolase 1 Antibody (Anti-ENO1)	Enzyme-linked immunosorbent assay for Antibody Detection.
SCB449Hu	CLIA Kit for Enolase 1 (ENO1)	Chemiluminescent immunoassay for Antigen Detection.