Biotin-Linked Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)

COL4-A5; ASLN; ATS; CA54; Collagen Alpha-5(IV)chain; Alport Syndrome

Product No.LAC141Hu71
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceAntibody labeling
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelBiotin
Original Antibody n/a
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
Downloadn/a
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116 270 US$ 270 US$ 386 US$ 965 US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL4a5. It has been selected for its ability to recognize COL4a5 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Experimental and Molecular Pathology	Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909
American Journal of Respiratory Cell and Molecular Biology	Basement Membrane Remodelling Controls Endothelial Function in IPAH Pubmed: 32160015

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC141Hu01	Recombinant Collagen Type IV Alpha 5 (COL4a5)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC141Hu01	Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)	WB;ICC/IF
LAC141Hu71	Biotin-Linked Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)	WB; IHC; ICC.
MAC141Hu21	Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)	WB; IHC; ICC; IP.
MAC141Hu22	Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)	WB; IHC; ICC; IP.
LAC141Hu82	FITC-Linked Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)	WB; IHC; ICC; IF.
SEC141Hu	ELISA Kit for Collagen Type IV Alpha 5 (COL4a5)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC141Hu	Multiplex Assay Kit for Collagen Type IV Alpha 5 (COL4a5) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.