Multiplex Assay Kit for Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay)

URP; SRP; UCN-II; UCNII; Stresscopin-Related Peptide; Urocortin-related peptide

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-99 81
EDTA plasma(n=5) 81-104 95
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Urocortin 2 (UCN2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-90% 98-105% 93-101% 78-97%
EDTA plasma(n=5) 80-95% 95-103% 85-99% 97-104%
heparin plasma(n=5) 88-102% 80-95% 80-96% 96-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:UCN2) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Clin Chem. High-Sensitivity Sandwich ELISA for Plasma NT-proUcn2: Plasma Concentrations and Relationship to Mortality in Heart Failure. Pubmed:27127183
Clinica Chimica Acta Urocortins: Actions in health and heart failure pubmed:28887029
Cardiovascular Research Urocortin-2 improves right ventricular function and attenuates pulmonary arterial hypertension Pubmed:29584808
Journal of Neuroendocrinology Glucocorticoid withdrawal affects stress‐induced changes in urocortin 2 gene expression in the rat adrenal medulla and brain Pubmed:29604138
High Blood Press Cardiovasc Prev Evaluation of Serum Urocortin 2 Levels in Patients with Hypertension Pubmed: 31925709
CURRENT PROBLEMS IN CARDIOLOGY Urocortin-2 in acute heart failure: role as a marker of volume overload and pulmonary hypertension 33994037
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