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Monoclonal Antibody to Platelet Factor 4 (PF4)

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

  • Product No.MAA172Bo21
  • Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 119  US$ 279  US$ 398  US$ 995  US$ 3980 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against PF4. It has been selected for its ability to recognize PF4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats PubMed: 20601223
Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170
PLoS One Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation PubMed: PMC3608633
Diving and Hyperbaric Medicine Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression Pubmed:25311322
Journal of Functional Foods A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivo science:S1756464616300676
Plos one Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury pubmed:27583400
international journal of molecular sciences Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. pubmed:28272338
Experimental and Therapeutic Medicine Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability pubmed:28450960
Reproductive Toxicology Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats Pubmed: 30528429
INTERNATIONAL JOURNAL OF CARDIOLOGY Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation Pubmed: 30857843
Available at SSRN 3335365  Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
journal of biotechnology Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis Pubmed: 30927490
Data in Brief Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation
journal of thoracic and cardiovascular surgery Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers Pubmed: 32812629
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents Pubmed: 31533230
Thrombosis Research Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices Pubmed: 31838449
Nat Commun The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets 34099640
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPA172Bo01 Recombinant Platelet Factor 4 (PF4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA172Bo01 Polyclonal Antibody to Platelet Factor 4 (PF4) WB; IHC; ICC; IP.
MAA172Bo21 Monoclonal Antibody to Platelet Factor 4 (PF4) WB; IHC; ICC; IP.
SEA172Bo ELISA Kit for Platelet Factor 4 (PF4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA172Bo Multiplex Assay Kit for Platelet Factor 4 (PF4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.