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Monoclonal Antibody to Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

SPECIFITY

The antibody is a mouse monoclonal antibody raised against OC. It has been selected for its ability to recognize OC in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
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Experimental Biology and Medicine Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured mice Rsm: source
Archives of Oral Biology Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats ScienceDirect: S0003996912001021
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Life Sciences Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes PubMed: 23357248
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Basic & Clinical Pharmacology & Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source
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Journal of Cranio-Maxillofacial Surgery The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study Pubmed:24485270
Naunyn-Schmiedeberg's Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats Pubmed:25038619
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
American Diabetes Association Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopenia Pubmed:25633418
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Indian Journal of Orthopaedics N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits. pubmed:27512225
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Journal of Bone and Mineral Metabolism MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967
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Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X
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Life Sciences Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway Pubmed:29883721
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588
Royal Society of Chemistry Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances c7tb02620j
Molecular Medicine Reports Effects of constrained dynamic loading, CKIP‑1 gene knockout and combination stimulations on bone loss caused by mechanical unloading Pubmed:29956799
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International Journal of Clinical and Experimental Medicine Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization Pubmed: 30689288
Chemico-Biological Interactions Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080
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American Journal of Translational Research Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
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Journal of Innovations in Pharmaceutical and Biological Sciences Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A A dual functional bone‐defect‐filling material with sequential antibacterial and osteoinductive properties for infected bone defect repair Pubmed: 31173657
Polymers Use of Polyphenol Tannic Acid to Functionalize Titanium with Strontium for Enhancement of Osteoblast Differentiation and Reduction of Osteoclast Activity Pubmed: 31362449
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International Journal of Nanomedicine Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling Pathway Pubmed: 31632013
Molecular Therapy-Nucleic Acids AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209
International Journal of Environmental Research and Public Health Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats Pubmed: 31739465
Pharmacology MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal Pubmed: 31694033
Brazilian Journal of Medical and Biological Research Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals Pubmed: 31826180
Biomedicine & Pharmacotherapy Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone loss Pubmed: 31846839
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Theranostics EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis Pubmed: 32194868
Journal of Korean Neurosurgical Society Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy Pubmed: 32172552
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cells Pubmed: 32064734
CELL BIOCHEMISTRY AND FUNCTION HSP90 inhibitors strengthen extracellular ATP‐stimulated synthesis of interleukin‐6 in osteoblasts: Amplification of p38 MAP kinase Pubmed: 32567086
Cell Death & Disease QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism Pubmed: 32382069
Molecular Medicine Reports Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism Pubmed: 33000198
Food & Function Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the … Pubmed: 33140795
ADVANCED FUNCTIONAL MATERIALS A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration
Revista Argentina de Clínica Psicológica Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene
淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究
Chemical Engineering Journal Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances
Surfaces and Interfaces In vitro comparison of titanium surface conditioning via boron-compounds and sand-blasting acid-etching
J Bone Miner Res Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway 33950533
Biochemical Engineering Journal Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis 34233019
PHARMACEUTICAL BIOLOGY Effects of icariin on the fracture healing in young and old rats and its mechanism 34511043
Evid Based Complement Alternat Med Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway 34512780
ACS Appl Mater Interfaces Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic Nanomedicine Pubmed:35311248
Molecules Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells Pubmed:35684342
Journal of Exercise Science & Fitness Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young Females Pubmed:35096081
Journal of Bionic Engineering Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA471Hu01 Recombinant Osteocalcin (OC) Positive Control; Immunogen; SDS-PAGE; WB.
RPA471Hu02 Recombinant Osteocalcin (OC) Positive Control; Immunogen; SDS-PAGE; WB.
CPA471Hu21 OVA Conjugated Osteocalcin (OC) Immunogen; SDS-PAGE; WB.
CPA471Hu22 OVA Conjugated Osteocalcin (OC) Immunogen; SDS-PAGE; WB.
CPA471Hu23 OVA Conjugated Osteocalcin (OC) Immunogen; SDS-PAGE; WB.
CPA471Hu31 KLH Conjugated Osteocalcin (OC) Immunogen; SDS-PAGE; WB.
PAA471Hu02 Polyclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
PAA471Hu01 Polyclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
PAA471Hu08 Polyclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu24 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu25 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu27 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu29 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu26 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu28 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu21 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu23 Monoclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MAA471Hu22 Monoclonal Antibody to Osteocalcin (OC) WB,IHC
SEA471Hu ELISA Kit for Osteocalcin (OC) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA471Hu High Sensitive ELISA Kit for Osteocalcin (OC) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA471Hu Multiplex Assay Kit for Osteocalcin (OC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA471Hu01 ELISA Kit DIY Materials for Osteocalcin (OC) Main materials for "Do It (ELISA Kit) Yourself".