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Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)

Image (I)

DAB staining on IHC-P; Sample: Human Ovarian cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Image (II)

Figure. Western Blot; Sample: Recombinant ERK1, Human.

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Instruction Manual

Monoclonal-Antibody-to-Extracellular-Signal-Regulated-Kinase-1-(ERK1)-MAB357Hu22.pdf

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Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

Product No.MAB357Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)C3
Ig Isotype IgG2a Kappa
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB357Hu01-Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration1mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 125 293 US$ 293 US$ 418 US$ 1045 US$ 4180
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Graefe's Archive for Clinical and Experimental Ophthalmology	Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909
International Journal of Reproductive Medicine	Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317
Sci Rep	Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB357Hu01	Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB357Hu01	Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)	WB; IHC; ICC; IP.
CAB357Hu01	Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody	Loading Control of WB.
MAB357Hu22	Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)	WB; IHC; ICC; IP.
MAB357Hu21	Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)	WB; IHC; ICC; IP.
CAB357Hu22	Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Monoclonal Antibody	Loading Control of WB.
SEB357Hu	ELISA Kit for Extracellular Signal Regulated Kinase 1 (ERK1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB357Hu	Multiplex Assay Kit for Extracellular Signal Regulated Kinase 1 (ERK1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.