≡

Monoclonal Antibody to Topoisomerase I (TOP1)

TOPI; DNA Topoisomerase 1

  • Product No.MAC243Hu22
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 111  US$ 259  US$ 370  US$ 925  US$ 3700 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against TOP1. It has been selected for its ability to recognize TOP1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Electronic Journal of Biotechnology Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris science:S0717345816000233
Journal of Experimental & Clinical Cancer Research Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319
Tikrit Journal of Pure Science Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight
EurAsian Journal of BioSciences Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC243Hu01 Recombinant Topoisomerase I (TOP1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC243Hu01 Polyclonal Antibody to Topoisomerase I (TOP1) WB; IHC; ICC; IP.
MAC243Hu22 Monoclonal Antibody to Topoisomerase I (TOP1) WB; IHC; ICC; IP.
SEC243Hu ELISA Kit for Topoisomerase I (TOP1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC243Hu Multiplex Assay Kit for Topoisomerase I (TOP1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.