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Monoclonal Antibody to Ferrochelatase (FECH)

EPP; FCE; Protoheme Ferrolyase; Protoporphyria; Heme synthase; Protoheme ferro-lyase

  • Product No.MAC488Hu22
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 111  US$ 259  US$ 370  US$ 925  US$ 3700 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against FECH. It has been selected for its ability to recognize FECH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Free Radical Biology and Medicine Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH Pubmed:35697292
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC488Hu01 Recombinant Ferrochelatase (FECH) Positive Control; Immunogen; SDS-PAGE; WB.
PAC488Hu01 Polyclonal Antibody to Ferrochelatase (FECH) WB; IHC; ICC; IP.
MAC488Hu22 Monoclonal Antibody to Ferrochelatase (FECH) WB; IHC; ICC; IP.
SEC488Hu ELISA Kit for Ferrochelatase (FECH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC488Hu Multiplex Assay Kit for Ferrochelatase (FECH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PGC488Hu01 Primer Pair for Ferrochelatase (FECH) PCR