Packages (Simulation)

Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)

Image (I)

DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 20μg/ml Mouse Anti-Human CD3e Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 10µg/ml Mouse Anti-Human CD3e Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Image (II)

Western Blot; Sample: Jurkat cell lysate Primary Ab: 2μg/ml Mouse Anti-Human CD3e Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

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Instruction Manual

Monoclonal-Antibody-to-T-Cell-Surface-Glycoprotein-CD3-Epsilon-(CD3e)-MAD117Hu22.pdf

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Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)

T3E; TCRE; CD3-E Molecule,Epsilon(CD3-TCR Complex); T-cell surface antigen T3/Leu-4 epsilon chain

Product No.MAD117Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)C3
Ig Isotype IgG1 Kappa
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPD117Hu01-Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration1mg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 111 259 US$ 259 US$ 370 US$ 925 US$ 3700
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CD3e. It has been selected for its ability to recognize CD3e in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-3µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
APD117Hu61	Active T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	Cell culture; Activity Assays.
APD117Hu62	Active T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	Cell culture; Activity Assays.
EPD117Hu61	Eukaryotic T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	Positive Control; Immunogen; SDS-PAGE; WB.
RPD117Hu01	Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	Positive Control; Immunogen; SDS-PAGE; WB.
EPD117Hu62	Eukaryotic T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD117Hu01	Polyclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC; ICC; IP.
MAD117Hu22	Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC; ICC; IP.
MAD117Hu23	Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC; ICC; IP.
RAD117Hu21	Recombinant Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IF; ICC; IHC; IP; FCM.
MAD117Hu24	Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC; ICC; IP.
MAD117Hu25	Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC; ICC; IP.
MAD117Hu27	Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC; ICC; IP.
MAD117Hu21	Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IHC
RAD117Hu22	Recombinant Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)	WB; IF; ICC; IHC; IP; FCM.
FAD117Hu02	Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Monoclonal Antibody	FCM
FAD117Hu86	FITC-Linked Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Monoclonal Antibody	FCM
FAD117Hu52	APC-Linked Anti-T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Monoclonal Antibody	Flow cytometry.