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Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1)

CYP1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1

  • Product No.MAD295Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 120  US$ 280  US$ 400  US$ 1000  US$ 4000 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CYP1A1. It has been selected for its ability to recognize CYP1A1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats Pubmed:25733458
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,3 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
International Journal of Chemical and Natural Science Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone portal:uploads
International Journal of Pharma Sciences Chemopreventive Role of Curcumin in benzo(A)pyrene induced Lung Carcinogenesis in mice via-modulation of Bcl-2, p53, Caspase-3, Cyp1A1, COX-2 and antioxidant defense system in Lung tissues portal:uploads
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
Biochemistry (Mosc) Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells Pubmed:29738693
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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PAD295Ra01 Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1) WB; IHC; ICC; IP.
MAD295Ra21 Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1) WB; IHC; ICC; IP.
SED295Ra ELISA Kit for Cytochrome P450 1A1 (CYP1A1) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSD295Ra01 ELISA Kit DIY Materials for Cytochrome P450 1A1 (CYP1A1) Main materials for "Do It (ELISA Kit) Yourself".