Packages (Simulation)
Image (I)
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DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 10µg/ml Mouse Anti-Human SFRP4 Antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 30µg/ml Mouse Anti-Human SFRP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Uterus Tissue
Primary Ab: 30µg/ml Mouse Anti-Human SFRP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
AF488 staining on IF;
Sample: A549 cell
Primary Ab: 30µg/ml Mouse Anti-Human SFRP4 Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
Image (II)
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Figure. Western Blot; Sample: Recombinant SFRP4, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Heart lysate; Lane4: SKOV3 cell lysate
Primary Ab: 2µg/ml Mouse Anti-Human SFRP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected
Quality Guarantee
Instruction Manual
Certificate
- KO-Validated
Monoclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)
FRP-4; FRPHE; Frizzled protein, human endometrium
- Product No.MAF878Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)5#
- Ig Isotype IgG2b Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPF878Hu01-Recombinant Secreted Frizzled Related Protein 4 (SFRP4)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 111
US$ 259
US$ 370
US$ 925
US$ 3700
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Endocrine Journal | Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance PubMed: 26041556 |
| Horm Metab Res. | Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes Mellitus Pubmed:26882051 |
| Obesity (Silver Spring). | Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesity pubmed:27768254 |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans Pubmed:29427376 |
| Novel marker for gestational diabetes patent:US20180059122A1 | |
| British Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile Pubmed: 30375290 |
| American Journal of Cancer Research | SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinoma Pubmed: 30906634 |
| Diabetologia | Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic … Pubmed: 31011777 |
| PLoS One | First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus Pubmed: 33206702 |
| Gastric Cancer | SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence 33277667 |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop … |