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Monoclonal Antibody to Glutaminase (GLS)

GLS1; Glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase

  • Product No.MAJ026Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 120  US$ 280  US$ 400  US$ 1000  US$ 4000 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against GLS. It has been selected for its ability to recognize GLS in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats Pubmed:28861894
Journal of Affective Disorders Glutamatergic hypofunction in medication-free major depression: Secondary effects of affective diagnosis and relationship to peripheral glutaminase Pubmed:29544167
校刊 King's Research Portal Doi: 10.1016/j.jad.2018.02.059
bmc research notes The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPJ026Ra01 Recombinant Glutaminase (GLS) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ026Ra01 Polyclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
MAJ026Ra21 Monoclonal Antibody to Glutaminase (GLS) WB; IHC; ICC; IP.
SEJ026Ra ELISA Kit for Glutaminase (GLS) Enzyme-linked immunosorbent assay for Antigen Detection.