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Mini Samples ELISA Kit for Colony Stimulating Factor 1, Macrophage (MCSF)
CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 1
- Product No.MEA090Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.0pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 374
US$ 534
US$ 2403
US$ 4539
US$ 37380
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF).
No significant cross-reactivity or interference between Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 102 |
| EDTA plasma(n=5) | 81-101 | 97 |
| heparin plasma(n=5) | 81-89 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Colony Stimulating Factor 1, Macrophage (MCSF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 87-98% | 97-105% | 80-101% |
| EDTA plasma(n=5) | 84-97% | 78-89% | 91-98% | 80-93% |
| heparin plasma(n=5) | 87-101% | 82-94% | 86-94% | 80-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Brain Pathology | Microglia-Derived Macrophage Colony Stimulating Factor Promotes Generation of Proinflammatory Cytokines by Astrocytes in the Periventricular White Matter in the Hypoxic Neonatal Brain Wiley: source |
| Oncotarget | miR-1207-5p suppresses lung cancer growth and metastasis by targeting CSF1 pubmed:27107415 |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654 |
| BioMed Research International | Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡ 33376718 |