Packages (Simulation)

Polyclonal Antibody to Leptin Receptor (LEPR)

Image (I)

IHC Images
IHC Image
IHC Image
IHC Image
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse LEPR Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse LEPR Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse LEPR Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Uterus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse LEPR Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Western Blot; Sample: Rat Cerebrum lysate Primary Ab: 0.3μg/ml Rabbit Anti-Mouse LEPR Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Porcine Cerebrum lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Mouse LEPR Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Leptin-Receptor-(LEPR)-PAA083Mu01.pdf

Certificate

Polyclonal Antibody to Leptin Receptor (LEPR)

CD295; LEPR; OBR; LR

Product No.PAA083Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA083Mu01-Recombinant Leptin Receptor (LEPR)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreRattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 97 227 US$ 227 US$ 324 US$ 810 US$ 3240
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LEPR. It has been selected for its ability to recognize LEPR in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Hormones and Behavior	Effects of androgen and leptin on behavioral and cellular responses in female rats ScienceDirect: S0018506X11001735
Nutrition & Diabetes	C-Peptide and leptin system in dichorionic, small and appropriate for gestational age twins—possible link to metabolic programming? Pubmed: 32778645

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA083Mu02	Recombinant Leptin Receptor (LEPR)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA083Mu01	Recombinant Leptin Receptor (LEPR)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA083Mu01	Polyclonal Antibody to Leptin Receptor (LEPR)	WB; IHC
PAA083Mu02	Polyclonal Antibody to Leptin Receptor (LEPR)	WB; IHC; ICC; IP.
LAA083Mu71	Biotin-Linked Polyclonal Antibody to Leptin Receptor (LEPR)	WB; IHC; ICC.
SEA083Mu	ELISA Kit for Leptin Receptor (LEPR)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA083Mu	Multiplex Assay Kit for Leptin Receptor (LEPR) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.