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Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

JTK14; Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1; Tyrosine-protein kinase receptor Tie-1

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Tie1. It has been selected for its ability to recognize Tie1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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