Packages (Simulation)

Polyclonal Antibody to Protein Kinase C Eta (PKCh)

Image (I)

DAB staining on IHC-P; Sample: Mouse Cerebrum Tissue Primary Ab: 10µg/ml Rabbit Anti-Human PKCh Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human PKCh Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human PKCh Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant PKCh, Human.

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Instruction Manual

Polyclonal-Antibody-to-Protein-Kinase-C-Eta-(PKCh)-PAA233Hu01.pdf

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Polyclonal Antibody to Protein Kinase C Eta (PKCh)

PRKCH; PRKC-H; PKC-L; PRKCL; NPKC-Eta

Product No.PAA233Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA233Hu01-Recombinant Protein Kinase C Eta (PKCh)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; ICC; IHC-P; IHC-F; ELISA.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PKCh. It has been selected for its ability to recognize PKCh in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA233Hu01	Recombinant Protein Kinase C Eta (PKCh)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA233Hu01	Polyclonal Antibody to Protein Kinase C Eta (PKCh)	WB; ICC; IHC-P; IHC-F; ELISA.
MAA233Hu22	Monoclonal Antibody to Protein Kinase C Eta (PKCh)	WB; IHC; ICC; IP.