Packages (Simulation)

Polyclonal Antibody to Centromere Protein H (CENPH)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human CENPH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
IHC Image

Image (II)

Figure. Western Blot; Sample: Recombinant CENPH, Human.
Western Blot; Sample: Mouse RAW264.7 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human CENPH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type RAW264.7 cell lysate;
Lane 2: CENPH knockout RAW264.7 cell lysate;
Predicted MW: 28kd
Observed MW: 28kd
Primary Ab: 3µg/ml Rabbit Anti-Human CENPH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human CENPH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human CENPH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Centromere-Protein-H-(CENPH)-PAA265Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Centromere Protein H (CENPH)

CENP-H; Enp; PMF1; NNF1; MIND Kinetochore Complex Component,Homolog

Product No.PAA265Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA265Hu01-Recombinant Centromere Protein H (CENPH)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 94 220 US$ 220 US$ 314 US$ 785 US$ 3140
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CENPH. It has been selected for its ability to recognize CENPH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA265Hu01	Recombinant Centromere Protein H (CENPH)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA265Hu01	Polyclonal Antibody to Centromere Protein H (CENPH)	WB,IHC
PGA265Hu01	Primer Pair for Centromere Protein H (CENPH)	PCR