Packages (Simulation)

Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)

Image (I)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Endometrial cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Prostate Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: THP1 cell
Primary Ab: 20µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant PECAM1, Human.
Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Human Lung lysate; Lane3: Human Platelet lysate; Lane4: Rat Spleen lysate; Lane5: 3T3-L1 cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human PECAM1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Platelet-Endothelial-Cell-Adhesion-Molecule-(PECAM1)-PAA363Hu01.pdf

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Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)

CD31; EndoCAM; GPIIA'; PECA1

Product No.PAA363Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA363Hu01-Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PECAM1. It has been selected for its ability to recognize PECAM1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
BioMed research international	Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA363Hu02	Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA363Hu01	Recombinant Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	Positive Control; Immunogen; SDS-PAGE; WB.
APA363Hu61	Active Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	Cell culture; Activity Assays.
EPA363Hu61	Eukaryotic Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA363Hu01	Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	WB; IHC; ICC; IP.
LAA363Hu71	Biotin-Linked Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	WB; IHC; ICC.
MAA363Hu22	Monoclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	WB; IHC; ICC; IP.
SEA363Hu	ELISA Kit for Platelet/Endothelial Cell Adhesion Molecule (PECAM1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA363Hu	Multiplex Assay Kit for Platelet/Endothelial Cell Adhesion Molecule (PECAM1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.