Packages (Simulation)
Image (I)
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DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ACTN2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Western Blot; Sample: A673 cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human ACTN2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: A431 cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Human ACTN2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Actinin Alpha 2 (ACTN2)
Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein
- Product No.PAA376Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA376Hu01-Recombinant Actinin Alpha 2 (ACTN2)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 95
US$ 221
US$ 316
US$ 790
US$ 3160
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ACTN2. It has been selected for its ability to recognize ACTN2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA376Hu01 | Recombinant Actinin Alpha 2 (ACTN2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA376Hu01 | Polyclonal Antibody to Actinin Alpha 2 (ACTN2) | WB; IHC; ICC; IP. |
| MAA376Hu22 | Monoclonal Antibody to Actinin Alpha 2 (ACTN2) | WB; IHC; ICC; IP. |
| SEA376Hu | ELISA Kit for Actinin Alpha 2 (ACTN2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA376Hu | Multiplex Assay Kit for Actinin Alpha 2 (ACTN2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |