Packages (Simulation)

Polyclonal Antibody to Actinin Alpha 2 (ACTN2)

Image (I)

DAB staining on IHC-P; Samples: Mouse Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse ACTN2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant ACTN2, Mouse.
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: ACTN2 knockout Hela cell lysate;
Predicted MW: 104kDa
Observed MW: 104kDa
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Skeletal muscle lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Actinin-Alpha-2-(ACTN2)-PAA376Mu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Actinin Alpha 2 (ACTN2)

Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein

Product No.PAA376Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA376Mu01-Recombinant Actinin Alpha 2 (ACTN2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreHomo sapiens (Human)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 97 227 US$ 227 US$ 324 US$ 810 US$ 3240
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ACTN2. It has been selected for its ability to recognize ACTN2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA376Mu01	Recombinant Actinin Alpha 2 (ACTN2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA376Mu01	Polyclonal Antibody to Actinin Alpha 2 (ACTN2)	WB; IHC
LAA376Mu71	Biotin-Linked Polyclonal Antibody to Actinin Alpha 2 (ACTN2)	WB; IHC; ICC.
LAA376Mu81	FITC-Linked Polyclonal Antibody to Actinin Alpha 2 (ACTN2)	WB; IHC; ICC; IF.