Packages (Simulation)

Polyclonal Antibody to Protein Kinase R (PKR)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PKR Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PKR Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PKR Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure: FITC staining on IHC-P;
Sample: MCF7 cells.

Image (II)

Figure. Western Blot; Sample: Recombinant PKR, Human.
Figure. Western Blot; Sample: Lane1: Human MCF7 Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PKR Polyclonal Antibody (Catalog PAA520Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Protein-Kinase-R-(PKR)-PAA520Hu01.pdf

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Polyclonal Antibody to Protein Kinase R (PKR)

PRKR; PRK-R; EIF2AK2; EIF2AK1; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; P1/eIF-2A kinase

Product No.PAA520Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA520Hu01-Recombinant Protein Kinase R (PKR)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PKR. It has been selected for its ability to recognize PKR in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-5μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
APA520Hu01	Active Protein Kinase R (PKR)	Cell culture; Activity Assays.
RPA520Hu01	Recombinant Protein Kinase R (PKR)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA520Hu01	Polyclonal Antibody to Protein Kinase R (PKR)	WB; IHC; ICC; IP; FCM
MAA520Hu22	Monoclonal Antibody to Protein Kinase R (PKR)	WB; IHC; ICC; IP.
SEA520Hu	ELISA Kit for Protein Kinase R (PKR)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA520Hu	Multiplex Assay Kit for Protein Kinase R (PKR) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.