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Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CDKN2A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 0.75µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Samples: Human Hela cell;
Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant CDKN2A, Human.
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: HepG2 cell lysate;
Primary Ab: 0.05µg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
WB Image
WB Image
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human CDKN2A Polyclonal Antibody (Catalog # PAA794Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CDKN2A Polyclonal Antibody (Catalog PAA794Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Cyclin-Dependent-Kinase-Inhibitor-2A-(CDKN2A)-PAA794Hu01.pdf

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Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)

P16; ARF; CDK4I; CMM2; INK4; INK4a; MLM; MTS1; TP16; P14; P14ARF; P16INK4a; P19; Melanoma P16; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1

Product No.PAA794Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA794Hu01-Recombinant Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC/IF;FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CDKN2A. It has been selected for its ability to recognize CDKN2A in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Biochemical genetics	Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer pubmed:28421310
European Journal of Pharmaceutical Sciences	Development of core-shell nanocarrier system for augmenting piperine cytotoxic activity against human brain cancer cell line Pubmed:29597041
Clinical and Experimental Medicine	Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes Pubmed: 31732824
MOLECULAR AND CELLULAR BIOCHEMISTRY	Tumor suppressor genes are differentially regulated with dietary folate modulations in a rat model of hepatocellular carcinoma Pubmed: 32974833

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA794Hu01	Recombinant Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA794Hu01	Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)	WB; IHC; ICC/IF;FCM
FAA794Hu01	Anti-Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) Polyclonal Antibody	Flow cytometry.
MAA794Hu22	Monoclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)	WB; IHC; ICC; IP.
MEA794Hu	Mini Samples ELISA Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)	Enzyme-linked immunosorbent assay for Antigen Detection.
SEA794Hu	ELISA Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA794Hu	Multiplex Assay Kit for Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.