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Polyclonal Antibody to Heat Shock Protein 90kDa Beta 1 (HSP90b1)

HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1;94 kDa glucose-regulated protein; Endoplasmin

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HSP90b1. It has been selected for its ability to recognize HSP90b1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cell Stress and Chaperones The influence of high-fat, high-sugar diet and bariatric surgery on HSP70 and HSP90 plasma and liver concentrations in diet-induced obese rats Pubmed: 30840227
Biology-Basel Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy 33374530
Cell Death & Disease The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress 33483465
Front Med Four-protein model for predicting prognostic risk of lung cancer Pubmed:35266102
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