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Polyclonal Antibody to Vitronectin (VTN)

V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against VTN. It has been selected for its ability to recognize VTN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer IEEE: 6098563
PLoS ONE Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance PubMed: PMC3754973
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Analytical Biochemistry A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots Pubmed:29154788
Journal of Materials Chemistry B Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs Doi: 10.1039/C8TB02283F 
JOURNAL OF NEPHROLOGY Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts 33275196
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